Part:BBa_K1334014:Design

formate dehydrogenase(FDH) from Escherichia.coli DH5a with a Flag-tag

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1260
  Illegal BglII site found at 1499
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 955
  Illegal AgeI site found at 1774
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The sequence of FDH from E.coli contains a TGA,but in fact,it can be translated into Se-methylselenocysteine

Source

The enzyme itself comes from E.coli DH5a and we add a Flag-tag.